Engineered occluded apo-intermediate of LacY
نویسندگان
چکیده
منابع مشابه
Apo-intermediate in the transport cycle of lactose permease (LacY).
The lactose permease (LacY) catalyzes coupled stoichiometric symport of a galactoside and an H(+). Crystal structures reveal 12, mostly irregular, transmembrane α-helices surrounding a cavity with sugar- and H(+)- binding sites at the apex, which is accessible from the cytoplasm and sealed on the periplasmic side (an inward-facing conformer). An outward-facing model has also been proposed based...
متن کاملEvidence for an intermediate conformational state of LacY.
LacY mutant Cys154 → Gly exhibits a periplasmic-closed crystal structure identical to the WT, but is periplasmic-open in the membrane. The mutant hardly catalyzes transport, but binds galactosides from either side of the membrane with the same affinity and is resistant to site-directed proteolysis relative to the pseudo-WT. Site-directed alkylation was also applied to 11 single-Cys mutants in C...
متن کاملFlexible Gates Generate Occluded Intermediates in the Transport Cycle of LacY☆
The major facilitator superfamily (MFS) transporter lactose permease (LacY) alternates between cytoplasmic and periplasmic open conformations to co-transport a sugar molecule together with a proton across the plasma membrane. Indirect experimental evidence suggested the existence of an occluded transition intermediate of LacY, which would prevent leaking of the proton gradient. As no experiment...
متن کاملIntermediate view synthesis considering occluded and ambiguously referenced image regions
In this paper, we present an algorithm for synthesizing intermediate views from a single stereopair. The key contributions of this algorithm are the incorporation of scene assumptions and a disparity estimation confidence measure that lead to the accurate synthesis of occluded and ambiguously referenced regions. The synthesized views have been displayed on a multi-view binocular imaging system,...
متن کاملElectrophysiological characterization of LacY.
Electrogenic events due to the activity of wild-type lactose permease from Escherichia coli (LacY) were investigated with proteoliposomes containing purified LacY adsorbed on a solid-supported membrane electrode. Downhill sugar/H(+) symport into the proteoliposomes generates transient currents. Studies at different lipid-to-protein ratios and at different pH values, as well as inactivation by N...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 2018
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.1816267115